Photoacoustic visualization of diatom algae

Авторы

  • Cvjetinovic, J. 1
  • Salimon, A. I. 2
  • Novoselova, M. V. 1
  • Sapozhnikov, P. V. 3
  • Kalinina, O. Yu. 4
  • Korsunsky, A. M. 2, 5
  • Gorin, D. A. 1
  • 1 Center for Photonics and Quantum Materials, Skolkovo Institute of Science and Technology, 3 Nobelya Str., Moscow, 121205, Russia
    2 Center for Energy Science and Technology, Skolkovo Institute of Science and Technology, 3 Nobelya Str., Moscow, 121205, Russia
    3 Shirshov Institute of Oceanology of Russian Academy of Sciences, 36 Nakhimovsky Prospekt, Moscow, 117997, Russia
    4 Faculty of Geography, Lomonosov Moscow State University, 1 Leninskiye Gory, Moscow, 119991, Russia
    5 Department of Engineering Science, University of Oxford, Oxford, OX1 3PJ, United Kingdom

DOI:

https://doi.org/10.31951/2658-3518-2020-A-4-779

Ключевые слова:

Diatoms, algae, cyanobacteria, photoacoustic imaging, photoacoustics

Аннотация

Photoacoustic imaging is a rapidly maturing imaging modality in biological research and medicine. Its underlying principle is the photoacoustic effect. This method can also be used to study algae containing light-absorbing chromophores, which produce a photoacoustic signal under laser irradiation. Here, we demonstrate successful photoacoustic visualization of unicellular diatom algae called Karayevia amoena. These hierarchical micro/nano-structured organisms mixed with an agarose gel were excited by nanosecond pulsed green, 532 nm laser using a raster scanning optoacoustic mesoscopy (RSOM) approach. We observed a strong photoacoustic signal from diatom cells and showed that it decreases with decreasing diatom concentration. The signal originates from chlorophyll a and c and fucoxanthin. We also studied cyanobacteria to prove that the photoacoustic technique can be useful for visualizing this type of object as well. Photoacoustic imaging is a promising method for remote control of algae cultivation in bioreactors and their natural environment.

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Опубликован

07.09.2020

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